Ino and Ani

"Two of our most wonderful Sisters are gone forever. But if you hear my words — they won't have died in vain."

- Una

Ino and Ani are Sisters who died prior to the events of the game, and almost managed to ascend from the Void to the Upper Limit.

Ino
"Ino died... Do you know who else is still alive?"

- Uta during the Famine

Ino was a domineering Sister, and the first Sister.

She attempted Breakthrough after Color spoke to her — but failed, and was cast down into the Nightmare for it. In the Nightmare, Ino turned into a Brother.

Yani hears Ino's voice, telling her about the Hero, and she appears to Sister in a dream.

Ani
"Ani died. We're dying, one after the other."

- Una during the Famine

Ani was a clever Sister, and the third Sister.

She found out about Breakthrough after Color spoke to her, and was cast down into the Nightmare for it. In the Nightmare, Ani turned into a Brother.

Ani told two other Sisters about Breakthrough. Yani hears Ani's voice, telling her about the Hero and Breakthrough.

Una was Ani's servant, and is said to still hear her voice in the roar of her smithy.